RPMI Medium 1640 細(xì)胞培養(yǎng) solarbio

RPMI Medium 1640，含L-谷氨酰胺、酚紅，不含丙酮酸鈉、HEPES

紅細(xì)胞裂解液的配置方法

取100 μl抗凝全血，加2 ml應(yīng)用液3#，混勻15～20 s；加2 ml應(yīng)用液2#，混勻15～20 s；加4 ml應(yīng)用液1#，混勻15～20 s；300×g離心2 min，棄上清；收集沉淀，加PBS緩沖液，制成白細(xì)胞懸液，備用。（這種紅細(xì)胞裂解法滿足了白細(xì)胞流式細(xì)胞儀檢測分選要求，價格低廉，是一種簡便快捷、經(jīng)濟(jì)實(shí)用的白細(xì)胞純化分選方法。）

紅細(xì)胞裂解液的注意事項(xiàng)

1、本裂解液為無菌產(chǎn)品，分離細(xì)胞用于細(xì)胞培養(yǎng)時請注意無菌操作。

2、為了您的安全與健康，請穿實(shí)驗(yàn)服并戴一次性手套操作。

3、 儲存液儲存于2-8°C不能超過半年(六個月)。

4、使用時根據(jù)需要稀釋,每次用都應(yīng)用去離子水新鮮配制工作液,當(dāng)日用不完的可廢棄。

北京索萊寶科技有限公司為紅細(xì)胞裂解液生產(chǎn)廠家，貨號為R1010，常賣規(guī)格為100ml 、500ml。索萊寶生產(chǎn)的紅細(xì)胞裂解液是利用細(xì)胞內(nèi)外存在鹽離子濃度差而導(dǎo)致細(xì)胞膜脹破的原理來裂解無核紅細(xì)胞的。產(chǎn)品經(jīng)過無菌處理，主要用于經(jīng)酶消化分散的組織細(xì)胞的分離純化，淋巴細(xì)胞的分離純化以及組織細(xì)胞蛋白與核酸提取等實(shí)驗(yàn)中紅細(xì)胞的去除。

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RPMI Medium 1640 細(xì)胞培養(yǎng) solarbio